Tutorials (legacy)
16 videos • 7,362 views • by Dr. K' teaches
Want to design PCR primers? Looking for cloning tips? How about guides about commonly used software? All this and more!
1
Tutorial 1: Designing basic PCR primers
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2
Tutorial 2: Cloning Part 1 (Overview)
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3
Tutorial 2: Cloning Part 2 (Advanced PCR Primer Design)
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4
Tutorial 2: Cloning Part 3 (Tm check, agarose gel confirmation, PCR purification)
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5
Tutorial 2: Cloning Part 4 (Digestion, gel purification, ligation)
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6
Tutorial 2: Cloning Part 5 (Transformation, DNA extraction, test digestion)
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7
Tutorial 3: Basics of SeqBuilder
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8
Tutorial 4: QuikChange site-directed mutagenesis (Stratagene)
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9
Tutorial 5: Basics of Pymol Part 1 (downloading, color coding, saving)
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10
Tutorial 5: Basics of Pymol Part 2 (measuring tool, polar contacts, mutagenesis)
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11
Tutorial 5: Basics of Pymol Part 3 (deleting amino acid residues, forming a peptide bond)
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12
Tutorial 6: How to use SeqMan to check your DNA sequencing results
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13
Tutorial 7: How to use ApE (Free DNA editing program) Part 1 [Introduction]
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14
Tutorial 7: How to use ApE (Free DNA editing program) Part 2 [ORFs and Features]
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15
Tutorial 7: How to use ApE (Free DNA editing program) Part 3 [Restriction Enzymes]
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16
Tutorial 8: How to align sequences using Clustal Omega (Free tool)
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